| HS Code | 458208 |
| Product Name | Beating Enzymes |
| Category | Proteolytic enzyme dietary supplement |
| Primary Support | Cardiovascular and circulatory health |
| Active Enzymes | Nattokinase, serrapeptase, lumbrokinase, and other proteolytic enzymes |
| Mechanism Of Action | Degrades fibrin and supports healthy blood viscosity and microcirculation |
| Dosage Form | Vegetable capsules |
| Recommended Intake | Generally 1-2 capsules daily on an empty stomach, or as directed by a healthcare practitioner |
| Key Benefit | Supports arterial health, healthy blood pressure, and overall cardiovascular function |
| Important Consideration | Consult a healthcare provider before use, particularly when taking anticoagulant or antiplatelet medications |
| Storage Instruction | Store in a cool, dry place away from direct sunlight and keep out of reach of children |
As an accredited Beating Enzymes factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.
| Packing | Packaged in sealed 25 kg fiber drums with desiccant-lined inner bags to protect enzyme activity during storage and transport. |
| Container Loading (20′ FCL) | 20′ FCL: Beating Enzymes packed in sealed drums/pails on pallets, secured with dunnage, labeled, and protected from moisture. |
| Shipping | Beating Enzymes ship as non-hazardous, temperature-sensitive biochemicals. Packed in sealed, moisture-proof containers within insulated cartons to prevent degradation. Avoid extreme heat or freezing; maintain dry conditions. No special hazmat labeling required. Include valid SDS and documentation for customs. Transport via standard freight with proper ventilation. |
| Storage | Store Beating Enzymes in a cool, dry place between 2–8°C (35–46°F), away from direct sunlight and heat. Keep the container tightly sealed to prevent moisture absorption. Avoid freezing. Use clean, dry scoops to prevent contamination. Always check the expiration date and follow the manufacturer’s instructions for optimal enzyme activity and shelf life. |
| Shelf Life | Shelf life: typically 12 months if unopened, stored cool and dry. Avoid moisture and heat after opening. |
The highest-volume commercial application for endoglucanase-rich beating enzymes is the treatment of 100% old corrugated containers (OCC) ahead of double-disc refining at containerboard mills. In recycled fibre, hornification and residual starch/ink solids reduce accessible fibrillation sites; enzyme addition after fine screening and before the machine chest at 0.10–0.30 kg/t on oven-dried fibre hydrolyses amorphous surface cellulose without altering load-bearing crystalline cores. The reaction is maintained at pH 6.0–7.5, 45–55 °C, 3–6% consistency, and 45–90 min retention. Refiner audit data show that freeness measured per ISO 5267-1 increases by 30–80 mL CSF at constant specific edge load, or the same final freeness is reached with 10–25% lower refining energy. The enzyme-modified stock is refined at 0.8–1.2 Ws/m specific edge load and 80–120 kWh/t total specific energy, then diluted and formed on a gap former. Residual sodium hypochlorite above 0.1 ppm in process water deactivates the enzyme, so oxidising biocide dosing is separated by at least 30 min from enzyme injection. Food-contact recycled liner and medium are governed by FDA 21 CFR 176.260, EU 1935/2004, and BfR Recommendation XXXVI. The process boundary is dose-related: enzyme dose excursions above 0.4 kg/t or retention beyond 120 min at 55 °C can generate excessive fines and lower short-span compressive strength per ISO 9895 by 3–7%; mills therefore maintain SCT index above 24–28 N·m/g for medium and 28–32 N·m/g for liner. Terminal products include corrugating medium of 90–150 g/m², test liner of 120–200 g/m², and white-top linerboard made with a bleached virgin top ply.
When bleached eucalyptus kraft is refined to 28–35 °SR, the conventional response often enters a fibre-cutting zone where tensile index gains flatten and formation defects become more visible at the size press. Endoglucanase-rich beating enzyme is dosed into the bleached high-density storage tower at 0.05–0.15 kg/t on oven-dried pulp, at pH 5.0–6.0, 50–60 °C, and 4–5% consistency for 45–60 min before low-consistency refining. Laboratory evaluation per ISO 5264-1 shows that the PFI mill revolutions required to reach 30 °SR can be reduced from 5000 rev to 2500 rev, with tensile index per ISO 1924-2 maintained within ±2–4% and air permeance per ISO 5636-3 remaining higher than the untreated pulp at equal freeness. Mill-scale refiner measurements record specific energy savings of 8–18% when final freeness is controlled at 28–35 °SR. The refined stock is blended with precipitated calcium carbonate filler to 12–18% ash, formed on a fourdrinier, and surface-sized at a film press. Grade compliance includes REACH registration for the enzyme preparation and ISO 9706 for archival permanence where required; energy measurements are reported under an ISO 14001 monitoring plan. The practical upper limit is 0.20 kg/t; above this level tear index per ISO 1974 may decline by 3–6% due to over-fibrillation. Terminal products include 70–120 g/m² copy paper, offset printing paper, inkjet paper, and continuous stationery.
For a crescent former tissue machine running northern bleached softwood kraft and eucalyptus, uncontrolled refining of the long-fibre fraction increases tensile energy but collapses sheet bulk and raises vacuum load at the suction breast roll. Xylanase-containing beating enzyme is dosed into the mixed furnish after the machine chest at 0.05–0.20 kg/t, pH 5.5–7.0, 45–60 °C, 3–5% consistency, and 30–60 min contact time. The xylanase fraction removes surface xylan and increases water removal in the forming zone, reducing vacuum demand at the crescent former by 5–15%. Drainage measured per ISO 5267-1 shows a 2–5 °SR reduction at constant refining energy. The treated furnish is refined lightly in a conical refiner, diluted to 0.1–0.3% headbox consistency, formed on a crescent former, and dried on a Yankee cylinder. Food-contact tissue and towel are governed by FDA 21 CFR 176.170, EU 1935/2004, and BfR Recommendation XXXVI. The operational boundary is set by creping mechanics: if headbox fines content falls below 3–5% measured per TAPPI T 261, the adhesive film on the Yankee dryer receives fewer hydrogen-bonding sites and blade chatter amplitude increases; hemicellulase dose is therefore limited to ≤0.25 kg/t. Terminal products include 14–20 g/m² bath tissue, 16–22 g/m² facial tissue, 38–50 g/m² kitchen towel, and napkin grades.
Rotary suction formers producing food-service trays from a furnish blend of unbleached softwood kraft, bamboo chemi-thermomechanical pulp, and 10–20% OCC use beating enzymes after pulper dilution but before the machine chest. The objective is to raise the Schopper-Riegler value from 18–22 °SR to 26–32 °SR while preserving long-fibre fraction for tool inlet uniformity. Addition rate is 0.10–0.25 kg/t on oven-dried fibre at pH 6.5–7.5, 50–60 °C, and 45–75 min at 3–4% consistency. The enzyme-treated stock is then passed through a low-speed disc refiner with an increased plate gap of 0.05–0.10 mm, transferred to a suction forming mould at 0.2–0.5% consistency, and hot-pressed at 150–190 °C. The enzyme effect is verified by freeness per ISO 5267-1, burst strength per ISO 2758, and caliper coefficient of variation below 3% on the forming tool. Food-contact moulded fibre uses FDA 21 CFR 176.170, EU 1935/2004, and BfR Recommendation XXXVI; compostable claims are tested to EN 13432 and ASTM D6868-21. The process boundary is critical at the transfer section: excess depolymerisation above 0.30 kg/t reduces wet-web tensile before hot pressing and can cause transfer breaks at suction pick-off. Terminal products include food-service trays, takeaway containers, coffee cup lids, egg cartons, agricultural seedling pots, and protective electronic cushioning inserts.
If the furnish is over-refined in a Hollander beater, the resulting sheet loses air permeance and the bubble point per ASTM F316-03 shifts outside the specified range. Specialty filter grades made from high-purity cotton linters, abaca, or mixed hardwoods are therefore treated with endoglucanase-rich beating enzyme at 0.02–0.10 kg/t on oven-dried fibre, pH 5.0–6.5, and 45–50 °C for 30–45 min before the beater. The enzyme selectively trims surface fibrillation and reduces fines generation during the 60–120 min beating cycle, maintaining a freeness of 20–30 °SR while allowing a 5–12% increase in air permeance measured by ISO 5636-3. The stock is then sheeted on a cylinder or fourdrinier machine and may be impregnated with wet-strength resin. Industry compliance for analytical and food-contact filtration grades includes FDA 21 CFR 176.170, EU 1935/2004, and grammage control per ISO 536. Published data for this specific configuration is limited above 0.10 kg/t, so mill trials with bubble point verification are required before dose escalation. Terminal products include quantitative filter paper, tea bag tissue, vacuum cleaner bag base paper, and battery separator base paper.
On a modern folding boxboard machine producing liquid packaging board grades from a triple-layer headbox, the middle and back plies are often made from bleached hardwood and softwood kraft refined in separate lines. Beating enzyme is applied only to the middle-ply softwood fraction at 0.08–0.18 kg/t, pH 5.5–7.0, 45–55 °C, 4–5% consistency, and 45–60 min retention before conical refining. The treatment permits the refiner to operate at 0.8–1.4 Ws/m specific edge load with 6–12% lower no-load power draw for the targeted 22–28 °SR freeness. A key physical requirement is bending stiffness per ISO 2493, which is retained within ±3% because the long-fibre fraction is not over-cut. Food-contact compliance of the finished board is governed by FDA 21 CFR 176.170, EU 1935/2004, and GB 9685-2016; the enzyme preparation itself must meet REACH registration. The process boundary is ply-bond integrity: reduction of fine fibre below 5% in the middle ply may impair internal bond strength measured per TAPPI T 569, so the dose is trimmed when ply-bond falls below 150–200 J/m². Terminal products include 180–250 g/m² folding boxboard, aseptic liquid packaging base board, cupstock, and frozen food carton board.
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Beating Enzymes is a liquid enzyme preparation supplied under three model designations—BE-1500, BE-2500, and BE-4500—for use in paper and board stock preparation. The formulation contains endo-1,4-β-glucanase (EC 3.2.1.4) and endo-1,4-β-xylanase (EC 3.2.1.8) activities in an aqueous carrier with stabilizers and preservatives. BE-1500 is assigned to bleached kraft hardwood and softwood blends with ash content below 3 %. BE-2500 is assigned to recycled linerboard, corrugating medium, and neutral sulfite semichemical furnishes with ash content between 3 % and 10 %. BE-4500 is assigned to high-ash recycled packaging grades where calcium carbonate fillers exceed 10 % by mass on oven-dry fiber. The model number corresponds to the target endo-1,4-β-glucanase activity in U/g, but lot-specific activity is the governing specification on the certificate of analysis. Activity is stated in U/g where one unit liberates 1 μmol of reducing sugar per minute from carboxymethyl cellulose or birchwood xylan at pH 5.5 and 50 °C.
| Model | Declared endo-1,4-β-glucanase activity | Declared endo-1,4-β-xylanase activity | Recommended pH | Temperature limit | Density at 20 °C | Typical assignment |
|---|---|---|---|---|---|---|
| BE-1500 | ≥1,500 U/g | ≥500 U/g | 5.5–7.5 | 35–60 °C | 1.05–1.10 g/cm³ | Bleached kraft hardwood |
| BE-2500 | ≥2,500 U/g | ≥1,000 U/g | 5.5–8.0 | 35–65 °C | 1.06–1.12 g/cm³ | Recycled liner and medium |
| BE-4500 | ≥4,500 U/g | ≥1,800 U/g | 6.0–8.0 | 35–60 °C | 1.08–1.14 g/cm³ | High-ash recycled packaging |
Declared activity is measured by reducing-sugar liberation under the conditions given above. The supplier applies a release tolerance of ±10 % for activity. Viscosity at 25 °C is controlled to ensure pumpability with progressive cavity dosing pumps; measured viscosity is ≤150 mPa·s for BE-1500 and BE-2500, and ≤200 mPa·s for BE-4500. The product is supplied in 25 kg, 200 kg, and 1000 kg containers. If stock temperature is below 10 °C, the formulation may thicken; it should be warmed to 20 °C before dosing without exceeding 40 °C during thawing or temperature recovery. The product should not be diluted with process water above 40 °C because local thermal denaturation may occur at hot-spot surfaces.
Endo-1,4-β-glucanase activity cleaves accessible β-1,4-glycosidic linkages in amorphous cellulose domains on the fiber surface and within the outer secondary wall. This limited hydrolysis creates stress-concentration sites that reduce the mechanical energy required for internal fibrillation during refining. Endo-1,4-β-xylanase activity removes re-deposited xylan from recycled fiber surfaces and fines. The product does not have measurable cellobiohydrolase activity; therefore, progressive depolymerization of crystalline cellulose during extended hold times is not expected at the recommended dose. The result is not a reduction in bulk stock viscosity; the dose is selected to avoid measurable fiber length loss. Fiber length is monitored by ISO 16065-2:2014. At recommended dose, the change in length-weighted fiber length relative to untreated refined pulp is typically between −2 % and −4 %; published data for this specific configuration is limited.
In mill practice, Beating Enzymes BE-2500 is charged to high-density storage or mixing chests before refining at a dose of 0.02 % to 0.15 % on oven-dry fiber. Contact time must be 30 min to 90 min at 35 °C to 60 °C. Dosing below 0.02 % may not produce a detectable change in refiner load; dosing above 0.15 % can increase fines without further energy displacement. The enzyme is diluted with filtered process water at a ratio of 1:10 to 1:20 immediately before the dosing point to improve distribution. Diluted solution must be used within 4 h. The addition point should be located after broke addition and before the refiner, but not at the same point as cationic polymer or alum because low-pH microenvironments can reduce activity.
In bleached kraft hardwood fine-paper furnishes, BE-1500 is dosed after final bleaching and washing, before the virgin pulp storage chest. The recommended charge is 0.03 % to 0.08 % on oven-dry fiber with a contact time of 45 min at 50 °C. Control is based on refiner motor load at constant freeness measured by ISO 5267-1:1999. The enzyme addition point should be positioned so that no strong oxidant carry-over from bleaching remains; residual peroxide above 2 mg/L can reduce activity. Published data for this specific configuration is limited to mill trials and should not be extrapolated without laboratory furnish characterization.
Short contact time is the primary limitation. When stock travels from the pulper to the refiner in less than 20 min, conversion of accessible cellulose is incomplete and dose response becomes non-linear. Production-scale observations on a 26-inch double-disc refiner operating at a constant specific edge load of 1.0 W s/m to 1.5 W s/m show that a dedicated holding chest providing at least 45 min residence is required for stable response. In a parallel campaign with direct pulper-to-refiner transfer, the same dose gave reduced response. The enzyme is not consumed uniformly; fines and dissolved anionic trash adsorb a portion of the protein, reducing the active fraction available for fiber surface modification. Therefore, high-ash recycled furnishes require the BE-4500 model with higher xylanase activity to address xylan re-adsorption on calcium carbonate fines.
Compared with single-component endo-1,4-β-glucanase products, BE-2500 and BE-4500 include a controlled endo-1,4-β-xylanase fraction. Single-component cellulase formulations can require a higher dose to achieve the same fibrillatory effect, and overdosing can produce measurable fiber length loss. The BE-Series is specified to contain no measurable cellobiohydrolase activity; this avoids progressive depolymerization during extended hold times. Compared with cationic starch and glyoxalated polyacrylamide dry-strength resins, the enzyme acts before the refiner and does not supply wet-end dry-strength. Cationic starch increases tensile index by hydrogen bonding and electrostatic bridging; it does not reduce refiner energy. Glyoxalated polyacrylamide contributes dry strength through crosslinking and is sensitive to anionic trash. Beating Enzymes is not a substitute for these chemicals and should not be dosed as a retention aid. Tensile comparisons should be made per ISO 1924-2:2008 on sheets formed under controlled wet pressing.
The product is intended for industrial paper and board manufacturing. It is not intended for direct food-contact paper unless the final article is assessed under Regulation (EC) No. 1935/2004 and applicable national provisions. Store at 5 °C to 15 °C in sealed containers. Shelf life is 12 months from date of manufacture when stored as recommended. Avoid freezing; freezing may cause protein aggregation and loss of activity. Avoid contact with oxidising biocides, including sodium hypochlorite, chlorine dioxide, and peracetic acid. Residual oxidant concentrations above 1 mg/L as Cl₂ will denature the enzyme. Do not pre-mix with strongly cationic polyelectrolytes or alum at pH below 4.5. The preparation is not intended for direct discharge to biological wastewater treatment without prior inactivation because protein may contribute to foam.
| Operational parameter | Limit or range | Monitoring method |
|---|---|---|
| Stock pH | 5.5–8.0 | pH meter calibrated per ASTM E70-19 |
| Stock temperature | 35–65 °C | Process temperature transmitter |
| Contact time before refining | 30–90 min | Residence time distribution |
| Dose on oven-dry fiber | 0.02–0.15 % | Mass balance calculation |
| Fiber length change | −2 % to −4 % | ISO 16065-2:2014 |
| Tensile index retention | Report only | ISO 1924-2:2008 |
| Ash assignment thresholds | 3 %, 10 % | ISO 1762:2019 |
